Anti-MPO antibodies, human

Anti-MPO antibodies, human

Clone: REA491 | Dilution: 1:10
Clone REA491 recognizes the human myeloperoxidase (MPO) antigen, a heterotetrameric protein consisting of two 15 kDa light chains and two variable-weight glycosylated heavy chains bound to a prosthetic heme group. MPO produces hypochlorous acid from hydrogen peroxide and chloride anion during the neutrophil's respiratory burst. Furthermore, it oxidizes tyrosine to tyrosyl radical using hydrogen peroxide as an oxidizing agent. MPO is present in azurophilic granules of neutrophilic granulocytes and monocytes and is also expressed in some acute myeloid leukemia cells. It plays a major role in the oxygen-dependent microbicidal system of of polymorphonuclear leukocytes. The deficiency of this enzyme activity results in a marked microbicidal defect.
Additional information: Clone REA491 displays negligible binding to Fc receptors.

Alternative names


Technical specifications

  • Antigen: MPO
  • Clone: REA491
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: Myeloperoxidase
  • Entrez Gene ID: 4353
  • Molecular mass of antigen [kDa]: 66
  • Distribution of antigen: granulocytes, monocytes, neutrophils
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC
  • Selected references

    1. Morishita, K. et al. (1987) Molecular cloning and characterization of cDNA for human myeloperoxidase. J. Biol. Chem. 262(8): 3844-3851
    2. Papayannopoulos, V. et al. (2010) Neutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps. J. Cell Biol. 191(3): 677-697
    3. Manivannan, P. et al. (2014) Can threshold for MPO by flow cytometry be reduced in classifying acute leukaemia? A comparison of flow cytometric and cytochemical myeloperoxidase using different flow cytometric cut-offs. Haematologica : Epub ahead of print
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