Anti-MHC Class II (I-Ab) antibodies, mouse

Anti-MHC Class II (I-Ab) antibodies, mouse

Clone: REA528 | Dilution: 1:10
Clone REA528 recognizes the mouse MHC class II alloantigen I-Ab of H-2b bearing mouse strains. It also cross-reacts with the H-2k and H-2u haplotypes, but reactivity to other haplotypes like, for example, d, f, g7, p, q, r, and s has not been observed. MHC class II is expressed on antigen-presenting cells, such as dendritic cells, monocytes, macrophages, B cells in lymphoid and non-lymphoid tissue, thymic epithelial cells, and on subsets of hematopoietic progenitor cells in the bone marrow.
Additional information: Clone REA528 displays negligible binding to Fc receptors.

Alternative names

MHC class II IAb

Technical specifications

  • Antigen: MHC Class II (I-Ab)
  • Clone: REA528
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: MHC class II IAb
  • Entrez Gene ID: 14961
  • Molecular mass of antigen [kDa]: 27
  • Distribution of antigen: B cells, dendritic cells, hematopoietic stem cells, macrophages, monocytes, thymic epithelial cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, APC, Biotin
  • Selected references

    1. Cuenca, M. et al. (2016) Targeting of Ly9 (CD229) disrupts marginal zone and B1 B cell homeostasis and antibody responses. J. Immunol. 196: 726-737
    2. Gradehandt, G. et al. (1993) The I-Ab-restricted alloresponse of D10.G4.1 T cells is based on the recognition of an endogenous peptide. Immunology 78(4): 592-599
    3. Tobita, T. et al. (2003) A role for the P1 anchor residue in the thermal stability of MHC class II molecule I-Ab. Immunol. Lett. 85(1): 47-52
    4. Zhu, Y. et al. (2003) Crystal structure of MHC class II I-Ab in complex with a human CLIP peptide: prediction of an I-Ab peptide-binding motif. J. Mol. Biol. 326(4): 1157-1174
Product options: 6
9 µg in 300 µL (1)
USD 79.00
30 µg in 1 mL (1)
USD 164.00
9 µg in 300 µL (1)
USD 50.00
30 µg in 1 mL (1)
USD 76.00
9 µg in 300 µL (1)
USD 26.00
30 µg in 1 mL (1)
USD 50.00

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