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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with CD3 and CD28 antibodies in the presence of IL-2 and IL-4 for 2 days. Cells were washed, expanded in the presence of IL-2 and IL-4 for 3 days, and restimulated with 5 ng/mL phorbol 12-myristate 13-acetate (PMA), 500 ng/mL ionomycin, and 1 µg/mL brefeldin A for 5 hours. Cells were then fixed, permeabilized, and stained with Anti-LIF antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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