Anti-IL-9 antibodies, human

Anti-IL-9 antibodies, human

Clone: REA1038 | Dilution: 1:50
Clone REA1038 recognizes the human interleukin 9 (IL-9) antigen, which is secreted by mast cells and T helper (Tʜ) cells. First IL-9 production was associated to Tʜ2 cells, but it was also found in other Tʜ subsets. Recently, evidence for the existence of a specialized lineage of Tʜ cells producing IL-9, named Tʜ9, came up. IL-9 has pleiotropic effects, e.g. it promotes mast cell growth and function, is involved in CD4
+
T cell subset differentiation and growth, enhances IgE production, supports hematopoiesis, and acts on airway and gut epithelial cells.
Additional information: Clone REA1038 displays negligible binding to Fc receptors.

Alternative names

HP40, P40

Technical specifications

  • Antigen: IL-9
  • Clone: REA1038
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: HP40, P40
  • Entrez Gene ID: 3578
  • Molecular mass of antigen [kDa]: 14
  • Distribution of antigen: mast cells, T helper cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC

  • Figure 1

    Human peripheral blood mononuclear cells (PBMCs) were isolated with the CD4
    +
    T Cell Isolation Kit, followed by a CD25 /CD45RO depletion. Afterwards, cells were either left unstimulated (left images) or stimulated with CD3 and CD28 antibodies as well as with human IL-4 and human IL-2 for three days. Cells were then polarized with human IL-4 and human IL-2 and restimulated with PMA and ionomycin. After two hours brefeldin A was added.
    Cells were fixed, permeabilized, and intracellularly stained with Anti-IL-9 antibodies as well as with CD4 and CD154 antibodies. CD4
    +
    cells were pre-gated for the analysis. Cells were then analyzed by flow cytometry using the MACSQuant
    ®
    Analyzer. Cell debris were excluded from the analysis based on scatter signals.
Product options: 4
130-117-586

Anti-IL-9-PE, human

for 30
tests
(1)
USD 102.00
130-117-437

Anti-IL-9-PE, human

for 100
tests
(1)
USD 241.00
130-117-587

Anti-IL-9-APC, human

for 30
tests
(1)
USD 122.00
130-117-438

Anti-IL-9-APC, human

for 100
tests
(1)
USD 288.00

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