Anti-IL-5 antibodies, mouse

Anti-IL-5 antibodies, mouse

Clone: TRFK-5 | Dilution: 1:10
Interleukin 5 (IL-5) is pre-dominantly secreted by CD4
+
T cells. It is involved in a range of allergic reactions and mediates immune reactions against parasites. IL-5 also acts on other cell types such as B cells.
The Anti-IL-5 antibody has been designed for intracellular staining of IL-5–producing cells. Cells can be stimulated for IL-5-production, for example, by polyclonal stimulation with mitogens. For induction of IL-5 production by antigen-specific T cells, cells are restimulated with respective antigen. IL-5 can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IL-5–producing cells can be stained intracellularly with Anti-IL-5 antibodies. Staining of surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the IL-5–producing cells. Magnetically enriched cells can be stained intracellularly for IL-5 production directly on the MACS
®
Column. This procedure ensures higher sensitivity of detection and minimizes loss of cells during washing procedures. The protocol is useful for cytokine analysis of rare cells, for example, CD4
+
T cells in HIV patients, or other cell sources than peripheral blood mononuclear cells (PBMCs), e.g., bronchoalveolar lavages or synovial fluids.

Technical specifications

  • Antigen: IL-5
  • Clone: TRFK-5
  • Isotype: rat IgG1κ
  • Isotype control: Rat IgG1 – isotype control antibodies
  • Entrez Gene ID: 16191
  • Molecular mass of antigen [kDa]: 13
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC, PE

  • Figure 1

    Mouse spleen cells were incubated with or without PMA/Ionomycin for 6 hours. After 2 hours, brefeldin A was added. The cells were harvested, fixed, permeabilized, and intracellularly stained with Anti-IL-5-PE, CD4-FITC, and CD154-APC. Cells were analyzed by flow cytometry using the MACSQuant® Analyzer. Gating was performed according to the CD4 expression and side scatter properties of the cells.
    Stimulated sample
    Unstimulated control
Product options: 2
130-102-434

Anti-IL-5-PE, mouse

30 µg in 1 mL (1)
USD 50.00
130-102-397

Anti-IL-5-APC, mouse

30 µg in 1 mL (1)
USD 81.00

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