Anti-IL-2 antibodies, human

Technical specifications

• Antigen: IL-2
• Clone: N7.48 A
• Isotype: mouse IgG2aκ
• Isotype control: Mouse IgG2a – isotype control antibodies
• Alternative names of antigen: TCGF, Lymphokine
• Entrez Gene ID: 3558
• Molecular mass of antigen [kDa]: 15
• Cross-reactivity:
  rhesus monkey (

  Macaca mulatta

  )
  ,
  cynomolgus monkey (

  Macaca fascicularis

  )
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE, APC, Vio515, Vio667

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were either unstimulated (left images) or stimulated with SEB for six hours. After two hours, brefeldin A was added. The cells were fixed, permeabilized, and intracellularly stained with Anti-IL-2 antibodies as well as with CD4 (Vit4) antibodies and CD69 antibodies. Cells were then analyzed by flow cytometry using the MACSQuant ® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. CD4 + lymphocytes were pre-gated for the analysis. Cell debris were excluded from the analysis based on scatter signals.

• Figure 2

  Rhesus monkey PBMCs were incubated with or without SEB for six hours. After two hours, brefeldin A was added. The cells were fixed, permeabilized, and intracellularly stained with Anti-IL-2-APC. Cells were analyzed by flow cytometry. Plots show CD4 + lymphocytes gated according to CD4-PE staining.

  Unstimulated control	Stimulated sample

• Figure 3

  Cynomolgus monkey PBMCs were incubated with or without SEB for six hours. After two hours, brefeldin A was added. The cells were fixed, permeabilized, and intracellularly stained with Anti-IL-2-PE. Cells were analyzed by flow cytometry.

  Unstimulated control	Stimulated sample