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Peritoneal exudate cells from BALB/c mice, either left unstimulated (left peak) or stimulated for 2 hours with LPS and IFN-γ followed by an overnight incubation with brefeldin A, were stained with Anti-F4/80 antibodies. Cells were then fixed, permeabilized, stained with Anti-IL-1a antibodies, and analyzed by flow cytometry using the MACSQuant®
cells were pregated for the analysis. Cell debris were excluded from the analysis based on scatter signals.
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