Anti-IL-17F antibodies, human

Anti-IL-17F antibodies, human

Clone: REA1012 | Dilution: 1:50
Clone REA1012 recognizes the human interleukin 17F (IL-17F) antigen, a homodimeric, 34 kDa member of the IL-17 family, which includes IL-17A, IL-17B, IL-17C, IL-17D, and IL-17E (also called IL-25). Interleukin 17 functions as a proinflammatory cytokine that responds to the invasion of the immune system by extracellular pathogens and induces destruction of the pathogen’s cellular matrix. Phylogenetic analysis reveals that among IL-17 family members, the IL-17F isoforms 1 and 2 (ML-1) have the highest homology to IL-17A (sharing 55 and 40% amino acid identity to IL-17A). IL-17F has been shown to have a pro-inflammatory role in asthma. It is expressed in the airway of asthmatics and its expression level is correlated with disease severity. IL-17F is able to induce several cytokines, chemokines and adhesion molecules in bronchial epithelial cells, vein endothelial cells, fibroblasts, and eosinophils. It is derived from several cell types such as T helper 17 (Tʜ17) cells, mast cells and basophils, and shows a wide tissue expression pattern including lung. Overexpression of the IL-17F gene in the airway of mice is associated with airway neutrophilia, the induction of many cytokines, an increase in airway hyperreactivity, and mucus hypersecretion. Hence, IL-17F may have a crucial role in allergic airway inflammation, and have important therapeutic implications in asthma.
Additional information: Clone REA1012 displays negligible binding to Fc receptors.

Alternative names

Interleukin-17F, CANDF6, ML-1

Technical specifications

  • Antigen: IL-17F
  • Clone: REA1012
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: Interleukin-17F, CANDF6, ML-1
  • Entrez Gene ID: 112744
  • Molecular mass of antigen [kDa]: 15
  • Distribution of antigen: granulocytes, mast cells, T cells, basophils
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, Vio667

  • Figure 1

    CD4
    +
    T cells were isolated from human peripheral blood mononuclear cells (PBMCs). Cells were then stimulated with 50 ng/mL Phorbol 12-myristate 13-acetate (PMA) and 1 µg/ml Ionomycin for 6 hours and then positively selected for their IL-17A expression. After cultivation for 11 days, cells were restimulated with 50 ng/mL PMA and 1 µg/ml Ionomycin for 6 hours, followed by an incubation with 1 µg/ml Brefeldin A for 4 hours. Cells were then fixed, permeabilized and stained with the REA Control (I) antibodes (left image) or with Anti-IL-17F antibodies. Flow cytometry was performed with the MACSQuant
    ®
    Analyzer. Cell debris were excluded from the analysis based on scatter signals.

  • Selected references

    1. Starnes, T. et al. (2001) Cutting edge: IL-17F, a novel cytokine selectively expressed in activated T cells and monocytes, regulates angiogenesis and endothelial cell cytokine production. J. Immunol. 167(8): 4137-4140
    2. Yang, X. O. et al. (2008) Regulation of inflammatory responses by IL-17F. J. Exp. Med. 205(5): 1063-1075
    3. Kawaguchi, M. et al. (2009) Role of interleukin-17F in asthma. Inflamm. Allergy Drug Targets 8(5): 383-389
Product options: 8
130-116-988

Anti-IL-17F-FITC, human

for 30
tests
(1)
USD 82.00
130-116-868

Anti-IL-17F-FITC, human

for 100
tests
(1)
USD 242.00
130-116-989

Anti-IL-17F-PE, human

for 30
tests
(1)
USD 104.00
130-116-869

Anti-IL-17F-PE, human

for 100
tests
(1)
USD 278.00
130-116-990

Anti-IL-17F-APC, human

for 30
tests
(1)
USD 124.00
130-116-870

Anti-IL-17F-APC, human

for 100
tests
(1)
USD 288.00
130-116-991

Anti-IL-17F-Vio667, human

for 30
tests
(1)
USD 166.00
130-116-871

Anti-IL-17F-Vio667, human

for 100
tests
(1)
USD 335.00

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