Anti-IFN-γ antibodies, human

Anti-IFN-γ antibodies, human

Clone: 45-15 | Dilution: 1:50
Interferon gamma (IFN-γ) is produced by cells that are involved in inflammatory immune responses. IFN-γ is predominantly released by memory and effector CD4
+
and CD8
+
T cells as well as by NK cells upon activation. Quantitative analysis of IFN-γ–producing cells can provide important information on the course of immune responses. Anti-IFN‑γ antibodies are designed for intracellular staining of IFN‑γ–producing cells. Cells can be stimulated for IFN-γ–production, e.g. by polyclonal stimulation with mitogens. For induction of IFN‑γ–production by antigen-specific T cells, cells are restimulated with respective antigen. IFN‑γ can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IFN‑γ–producing cells can be stained intracellularly with Anti-IFN‑γ antibodies. Staining of surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the IFN-γ–producing cells.

Alternative names

IFNg, IFG, IFI

Technical specifications

  • Antigen: IFN-γ
  • Clone: 45-15
  • Isotype: mouse IgG1κ
  • Isotype control: Mouse IgG1 – isotype control antibodies
  • Alternative names of antigen: IFNg, IFG, IFI
  • Entrez Gene ID: 3458
  • Molecular mass of antigen [kDa]: 16
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, PE-Vio770, APC-Vio770

  • Figure 1

    Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with SEB for six hours. After two hours, brefeldin A was added for stimulated and unstimulated cells. The cells were fixed, permeabilized, and intracellularly stained with Anti-IFN-γ antibodies as well as with CD4 antibodies and CD69 antibodies. Cells were then analyzed by flow cytometry using the MACSQuant
    ®
    Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. CD4
    +
    lymphocytes were pre-gated for the analysis. Cell debris were excluded from the analysis based on scatter signals.

  • Figure 2

    Rhesus monkey PBMCs were incubated with (left image) or without SEB for six hours. After two hours, brefeldin A was added. The cells were fixed, permeabilized, and intracellularly stained with Anti-IFN-γ antibodies as well as with CD4 antibodies and CD69 antibodies. Cells were analyzed by flow cytometry. Plots show CD4
    +
    lymphocytes gated according to CD4-APC staining.

  • Figure 3

    Cynomolgus monkey PBMCs were incubated with (left image) or without SEB for six hours. After two hours, brefeldin A was added. The cells were fixed, permeabilized, and intracellularly stained with Anti-IFN-γ antibodies and CD4 antibodies. Cells were analyzed by flow cytometry.
Product options: 10
130-114-018

Anti-IFN-γ-FITC, human

for 30
tests
(1)
USD 82.00
130-113-492

Anti-IFN-γ-FITC, human

for 100
tests
(1)
USD 242.00
130-114-019

Anti-IFN-γ-PE, human

for 30
tests
(1)
USD 104.00
130-113-493

Anti-IFN-γ-PE, human

for 100
tests
(1)
USD 278.00
130-114-016

Anti-IFN-γ-APC, human

for 30
tests
(1)
USD 124.00
130-113-490

Anti-IFN-γ-APC, human

for 100
tests
(1)
USD 288.00
130-114-020

Anti-IFN-γ-PE-Vio770, human

for 30
tests
(1)
USD 148.00
130-113-494

Anti-IFN-γ-PE-Vio770, human

for 100
tests
(1)
USD 320.00
130-114-017

Anti-IFN-γ-APC-Vio770, human

for 30
tests
(1)
USD 138.00
130-113-491

Anti-IFN-γ-APC-Vio770, human

for 100
tests
(1)
USD 325.00

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