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Immunofluorescence analysis of HA-BDCA-2 expressing rat basophilic leukaemia-1 (RBL-1) cells. RBL-1 cells transiently expressing HA-BDCA-2 were analyzed by confocal laser scanning microscopy (CLSM) 24 hours after transfection. Cells were first labeled with Anti-BDCA-2-Biotin, fixed, permeabilized, and stained with Anti-HA-FITC and Streptavidin-Alexa633, respectively.A
) Cell surface and intracellular staining of BDCA-2 in transfected RBL-1 cells.B
) Overlay of transmission and CLSM image.