Anti-CLA antibodies, human

Anti-CLA antibodies, human

Clone: HECA-452 | Dilution: 1:11
The Anti-CLA antibody recognizes the cutaneous lymphocyte-associated antigen (CLA), a specialized glycoform of P-selectin glycoprotein ligand-1 (PSGL-1). It serves as a ligand for selectins, including CD62E (ELAM-1) and CD62L (LECAM-1). CLA is a unique skin-homing receptor and is predominantly found on a minor subset of human T cells that infiltrate the skin. This post-translational modification of PSGL-1 is thought to serve as a mechanism to regulate tissue-specific homing of CD4
+
and CD8
+
memory/effector T cells from peripheral blood to the skin, which plays an essential role during many inflammatory and certain malignant skin diseases.
In peripheral blood, CLA is not only found on skin-homing 
memory/effector T cells, but is also found to be expressed on memory/effector B cells, NK cells, blood dendritic cells, and on monocytes. CLA is furthermore found on Langerhans cells in the skin.

Alternative names

SELPLG, CD162, PSGL-1, PSGL1

Technical specifications

  • Antigen: CLA
  • Clone: HECA-452
  • Isotype: rat IgMκ
  • Isotype control: Rat IgM – isotype control antibodies
  • Alternative names of antigen: SELPLG, CD162, PSGL-1, PSGL1
  • Entrez Gene ID: 6404
  • Molecular mass of antigen [kDa]: 39
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, PE-Vio770, APC-Vio770, Biotin, pure
  • Selected references

    1. Fuhlbrigge, R. C. et al. (1997) Cutaneous lymphocyte antigen is a specialized form of PSGL-1 expressed on skin-homing T cells. Nature 389: 978-981
    2. Santamaria Babi, L. F. et al. (1995) Migration of skin-homing T cells across cytokine-activated human endothelial cell layers involves interaction of the cutaneous lymphocyte-associated antigen (CLA), the very late antigen-4 (VLA-4), and the lymphocyte function-associated antigen-1 (LFA-1). J. Immunol. 154: 1543-1550
    3. Takahashi, R. et al. (2003)
      In vitro
      differentiation from naive to mature E-selectin binding CD4 T cells: acquisition of skin-homing properties occurs independently of cutaneous lymphocyte antigen expression.
      J. Immunol. 171: 5769-5777
    4. Yoshino, T. et al. (1999) Cutaneous lymphocyte antigen is expressed on memory/effector B cells in the peripheral blood and monocytoid B cells in the lymphoid tissues. Cell. Immunol. 197: 39-45
    5. Bos, J. D. et al. (1993) Skin-homing T lymphocytes: detection of cutaneous lymphocyte-associated antigen (CLA) by HECA.452 in normal human skin. Arch. Dermatol. Res. 285(179): 179-183
    6. Strunk, D. et al. (1997) A skin homing molecule defines the Langerhans cell progenitor in human peripheral blood. J. Exp. Med. 185: 1131-1136
    7. Tsuchiyama, J. et al. (2002) Induction and characterization of cutaneous lymphocyte antigen on natural killer cells. Br. J. Haematol. 118: 654-662
    8. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
Product options: 12
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USD 305.00
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USD 132.00
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tests
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USD 315.00
for 100
tests
(1)
USD 232.00
100 µg in 1 mL
USD 140.00

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