Name: Anti-c-myc antibodies
Availability: InStock

Product overview

The monoclonal Anti-c-myc antibody facilitates the fast and convenient detection of c-myc-tagged fusion proteins, expressed in either prokaryotic or eukaryotic cells. The antibody is available conjugated with horseradish peroxidase (HRP) or fluorochrome- or biotin-conjugated for fast and convenient analysis of c-myc-tagged fusion proteins. Conjugation of the Anti-c-myc antibody to HRP simplifies Western blot or ELISA analysis as the incubation with secondary antibodies becomes dispensable. After incubation the Anti-c-myc-HRP antibody can be directly detected using commercially available chemiluminescent reagents.

The monoclonal Anti-c-myc antibody specifically recognizes proteins that are tagged with the c-myc epitope (EQKLISEEDL). Fluorochrome-conjuagted Anti-c-myc antibodies enable direct flow cytometry and fluorescence microscopy analyses while indirect fluorescent labeling of c-myc fusion protein expressing cells is possible with the biotin-conjugated antibody.

Detailed product information

Technical specifications

Antigen: c-myc
Clone: SH1-26E7.1.3
Isotype: mouse IgG1
Isotype control: Mouse IgG1 – isotype control antibodies
Molecular mass of antigen [kDa]: 1.2
Distribution of antigen: eukaryotic cells, prokaryotic cells
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: Biotin, HRP

Product data and images

Figure 1

Immunofluorescence analysis of c-myc-BDCA-2 expressing CHO cells. CHO cells transiently expressing c-myc-BDCA-2 were analyzed by fluorescence microscopy 24 hours after transfection.

) Double staining of c-myc-BDCA-2 with Anti-c-myc-FITC and Anti-BDCA-2-Biotin/Anti-Biotin-Alexa 546.

) Double staining of c-myc-BDCA-2 with Anti-c-myc-Biotin/Anti-Biotin-Alexa 546 and BDCA-2-FITC. Red: Alexa 546-labeling, green: FITC-labeling, yellow: FITC-Alexa 546 overlay.

A:	B:

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Figure

Detection of transiently expressed c-myc fusion proteins using the Anti-c-myc-HRP antibody.

HEK 293 cells transiently expressing c-myc-BDCA-2 were lysed and c-myc-BDCA-2 was purified using the μMACS Anti-c-myc Isolation Kit. 1/100, 1/500, and 1/1000 of the eluate were subjected to SDS-polyacrylamide gel electrophoresis, blotted onto a PVDF membrane and detected using Anti-c-myc-HRP antibody (1:10,000, 1 h, room temperature) and ECL reagent.

Detection of transiently expressed c-myc fusion proteins using the Anti-c-myc-HRP antibody.

HEK 293 cells transiently expressing c-myc-BDCA-2 were lysed and c-myc-BDCA-2 was purified using the μMACS Anti-c-myc Isolation Kit. 1/100, 1/500, and 1/1000 of the eluate were subjected to SDS-polyacrylamide gel electrophoresis, blotted onto a PVDF membrane and detected using Anti-c-myc-HRP antibody (1:10,000, 1 h, room temperature) and ECL reagent.