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Human peripheral blood mononuclear cells (PBMCs) were stained with CD19 antibodies and fixed and permeabilized using the Cell Signaling Buffer Set A. Cells were then stained with Anti-Btk antibodies or with the corresponding REA Control (I) antibodies (left images) and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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