Anti-α-Actinin (Sarcomeric) antibodies, human, mouse, rat

Anti-α-Actinin (Sarcomeric) antibodies, human, mouse, rat

Clone: REA402 | Dilution: 1:10
Clone REA402 recognizes the sarcomeric α-actinin antigen, a 100 kDa actin-binding protein, which occupies a strategic role in the assembly and maintenance of stress fibers of non-muscle cells and the myofibrils of muscle cells. α-actinins have been highly conserved throughout evolution and are largely collinear proteins that share three conserved functional domains. Functionally, α-actinins form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. Two isoforms of α-actinin have been identified, a muscle-specific sarcomeric isoform and a non-sarcomeric isoform. The major – and best characterized – functional difference among α-actinin isoforms is calcium sensitivity for actin-binding. The binding of α-actinin to actin by non-muscle cytoskeletal isoforms is dependent on the calcium concentration, whereas this interaction is independent of calcium concentration in sarcomeric striated and smooth muscle isoforms. Studies on skeletal and cardiac cells have localized sarcomeric α-actinin to Z-bands in striated myofibrils, to precursor I-Z-I-like complexes in muscle undergoing myofibrillogenesis, and to vinculin-positive adhesion plaques and adherens junctions.
Additional information: Clone REA402 displays negligible binding to Fc receptors.

Alternative names

ACTN2, CMD1AA, CMH23

Technical specifications

  • Antigen: α-Actinin
  • Clone: REA402
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: ACTN2, CMD1AA, CMH23
  • Entrez Gene ID: 88
  • Molecular mass of antigen [kDa]: 104
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
    , sheep, rabbit, lizard, feline, canine, snake, fish, goat, chicken, bovine, frog, pig, hamster
  • Distribution of antigen: ES and iPS cells, other
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, VioBlue, Biotin, pure

  • Figure 1

    Heart tissue from P1 Wistar rats was dissociated using the Neonatal Heart Dissociation Kit and the gentleMACS™ Dissociator. Neonatal cardiomyocytes were then fixed, permeabilized, and stained with Anti-α-Actinin (Sarcomeric) antibodies or with the corresponding REA Control antibodies (left images). Flow cytometry was performed using the MACSQuant
    ®
    Analyzer. Cell debris were excluded from the analysis based on scatter signals.
Product options: 7
130-106-998

Anti-α-Actinin (Sarcomeric)-PE, human, mouse, rat

for 30
tests
(1)
USD 104.00
130-106-937

Anti-α-Actinin (Sarcomeric)-PE, human, mouse, rat

for 100
tests
(1)
USD 278.00
130-106-996

Anti-α-Actinin (Sarcomeric)-VioBlue, human, mouse, rat

for 30
tests
(1)
USD 130.00
130-106-935

Anti-α-Actinin (Sarcomeric)-VioBlue, human, mouse, rat

for 100
tests
(1)
USD 315.00
130-106-995

Anti-α-Actinin (Sarcomeric)-Biotin, human, mouse, rat

for 30
tests
(1)
USD 86.00
130-106-934

Anti-α-Actinin (Sarcomeric)-Biotin, human, mouse, rat

for 100
tests
(1)
USD 232.00
130-112-755

Anti-α-Actinin (Sarcomeric) pure, human, mouse, rat

100 µg in 100 µL
USD 130.00

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