StraightFrom® Whole Blood CD45 MicroBeads

StraightFrom
®
Whole Blood CD45 MicroBeads

StraightFrom
®
Whole Blood CD45 MicroBeads were developed for rapid positive selection of CD45
+
cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. No sample preparation is required, including density gradient centrifugation and erythrocyte lysis.

Background information

The CD45 antigen is expressed on all cells of hematopoietic origin except erythrocytes, platelets, and their precursor cells.

Detailed separation procedure

Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom
®
Whole Blood CD45 MicroBeads and subsequently separated with the autoMACS
®
Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit. StraightFrom Whole Blood MicroBeads in combination with the autoMACS Separators allow standardization of the cell separation procedure and safe handling of hazardous samples.

Downstream applications

The purified CD45
+
cells are well suited for flow cytometric, functional, or molecular analysis including chimerism analysis after allogeneic stem cell transplantation
1
.

Columns

autoMACS
®
Columns or Whole Blood Columns.
Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

Product options: 1
for 40
mL whole blood
USD 620.00

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