StraightFrom® Whole Blood CD4 MicroBeads, human

StraightFrom
®
Whole Blood CD4 MicroBeads
, human

StraightFrom
®
Whole Blood CD4 MicroBeads were developed for rapid automated positive selection of CD4
+
cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.

Background information

StraightFrom
®
Whole Blood CD4 MicroBeads recognize about 65% of all peripheral blood T cells. The CD4 antigen is expressed on T helper cells and at a lower level on monocytes and dendritic cells. The CD4 antigen is an accessory molecule in the recognition of MHC class II/peptide complexes by the TCR heterodimer on CD4
+
T helper cells.

Detailed separation procedure

Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom
®
Whole Blood CD4 MicroBeads to be subsequently separated with the autoMACS
®
Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Downstream applications

StraightFrom
®
Whole Blood MicroBeads allow the fast isolation of CD4
+
cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yields of target cells. StraightFrom Whole Blood MicroBeads in combination with the autoMACS
®
Pro Separator allow standardization of the cell separation procedure and safe handling of hazardous samples. The purified CD4
+
cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation
1,2
.

Columns

autoMACS
®
Columns or Whole Blood Columns.
  • Selected references

    1. Meyer, R. G. et al. (2007) Prophylactic transfer of CD8-depleted donor lymphocytes after T-cell-depleted reduced-intensity transplantation. Blood 109: 374-382
    2. Koehl, U. et al. (2003) Quantitative analysis of chimerism after allogeneic stem cell transplantation by PCR amplification of microsatellite markers and capillary electrophoresis with fluorescence detection: the Frankfurt experience. Leukemia 17: 232-236
    3. Adams, S. et al. (2004) Cell lineage-specific chimerism in posthematopoietic stem cell transplant patients. MACS&more 8(2): 16
  • Brochures and posters

Video

How to isolate immune cells directly from blood

For more information, visit https://bit.ly/2RJiJUd Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

Product options: 1
130-090-877

StraightFrom Whole Blood CD4 MicroBeads, human

for 40
mL whole blood
USD 620.00