StraightFrom® Whole Blood CD19 MicroBeads, human

StraightFrom
®
Whole Blood CD19 MicroBeads
, human

StraightFrom
®
Whole Blood CD19 MicroBeads allow the fast isolation of CD19
+
cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. No sample preparation is required, including density gradient centrifugation and erythrocyte lysis.

Background information

The CD19 antigen is expressed from the early pro–B cell to the B cell blast stage. It is also present on most malignant B cells. CD19 expression is down-regulated upon terminal differentiation of B cells to plasma cells.

Detailed separation procedure

Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom
®
Whole Blood CD19 MicroBeads and subsequently separated with the autoMACS
®
Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Downstream applications

The purified CD19
+
cells are well suited for flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation
1,2,3
.

Columns

autoMACS
®
Columns or Whole Blood Columns.
Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

Product options: 1
for 40
mL whole blood
USD 620.00

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