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StraightFrom® Leukopak® MicroBeads allow for the magnetic cell separation of large leukocyte numbers directly from Leukopak® (StemExpress, LCC). Leukocyte subsets such as CD4+, CD8+, CD14+, or CD19+ cells can be purified using either semi-or fully automated using MultiMACS™ Instruments. Pre-enrichment of white blood cells, e.g. by density gradient centrifugation, is not required. The purified cells are well-suited for further flow cytometric analyses, molecular biology applications, and functional studies.
CD14+ cells can be isolated straight from a Leukopak® sample using the StraightFrom Leukopak® CD14 MicroBead Kit, human and the MultiMACS™ Cell24 Separator Plus.
Separation of a Leukopak® sample using the StraightFrom Leukopak® CD14 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Multi-24 Column Block. Cells were fluorescently stained with CD14-APC, CD15-PE-Vio® 770, as well as CD45-VioBlue® and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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