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Mouse skeletal muscle was dissociated using the Skeletal Muscle Dissociation Kit, mouse and rat (# 130-098-305) in combination with the gentleMACS Dissociator. Subsequently, satellite cells were isolated using the Satellite Cell Isolation Kit, mouse and cultured in expansion medium (40% DMEM, 40% HAM’s F10, 2.5 ng/mL human FGF-2, 100 U/mL penicillin/ 100 U/mL streptomycin) for six days.
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