Name: MACSprep™ PBMC Isolation Kit, human
Availability: InStock

Product overview

The MACSprep™ PBMC Isolation Kit, human has been developed for the fast isolation of human peripheral blood mononuclear cells (PBMCs) from 1–8 mL of freshly drawn anticoagulated whole blood without density gradient centrifugation.

Detailed product information

Background information

The isolation of human peripheral blood mononuclear cells (PBMCs) is performed with only one labeling step and in a two-step separation procedure. During the first isolation step erythrocytes are aggregated and sedimented. In a second step, PBMCs are isolated by depletion of non-PBMCs (e.g. neutrophils, eosinophils, platelets, and residual erythrocytes). Non-PBMCs are indirectly magnetically labeled with a cocktail of biotin-conjugated monoclonal antibodies and MACSprep™ Anti-Biotin MicroBeads. The magnetically labeled non-PBMCs are depleted by retaining them within a MACS Column in the magnetic field of a MACS

^®

Separator while the unlabeled PBMCs run through.

Downstream applications

The MACSprep™ PBMC Isolation Kit, human is used for the fast isolation of human peripheral blood mononuclear cells (PBMCs).

Applications include:

Efficient, fast, and convenient isolation of untouched PBMCs from blood samples of 1–8 mL
PBMC co-cultures, e.g., functional immune cell assays
Studies on cytokine expression of immune cells upon restimulation
Studies on signal transduction during activation of immune cells
Immune monitoring of antigen-specific T cells

Product data and images

Figure 1

Untouched PBMCs were isolated from 10 mL of human EDTA-anticoagulated whole blood using the MACSprep™ PBMC Isolation Kit, a MACSmix™ Tube Rotator, and an LS Column. The isolated cells were fluorescently stained with CD45-VioBlue

^®

, CD3-FITC, CD4-VioGreen™, CD8-APC-Vio

^®

 770, CD14-APC, CD16-PE, CD56-PE, CD20-PE-Vio770, and 7-AAD and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and 7-AAD.

Before separation

Lysed whole blood gated on CD45 ⁺ cells

After separation

Gated on CD45 ⁺ cells	Gated on viable CD45 ⁺ cells	Gated on viable CD45 ⁺ cells

Gated on viable CD45 ⁺ CD3 ⁺ cells	Gated on viable CD45 ⁺ CD14 ⁺ cells

Product options: 1

130-115-169