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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
A tumor induced by the B16-OVA cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD8 + TILs were isolated from the single-cell suspension using CD8 (TIL) MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD8b-VioBlue ® and CD4-PE-Vio ® 615 and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and Viobility™ 405/520 Fixable Dye fluorescence. |
Before separation | CD8 + cells |
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