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A tumor induced by the B16-OVA cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD8 + TILs were isolated from the single-cell suspension using CD8 (TIL) MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD8b-VioBlue ® and CD4-PE-Vio ® 615 and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and Viobility™ 405/520 Fixable Dye fluorescence. |
Before separation | CD8 + cells |
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