Name: CD45 MicroBeads, human
Availability: InStock

Product overview

CD45 MicroBeads were developed for the positive selection or depletion of leukocytes from peripheral blood, erythrocyte and thrombocyte preparations, lymphoid tissues, as well as nonhematopoietic tissues.

Detailed product information

Background information

The CD45 antigen is expressed on all cells of hematopoietic origin except erythrocytes, platelets, and their precursor cells.

Applications

CD45 MicroBeads have been used to enrich fetal erythroblasts from maternal blood. They were also used to enrich epithelial tumor cells from peripheral blood, bone marrow, or lymphoid tissue by depletion of CD45

⁺

leukocytes

. Stem cells from human bone marrow have been enriched by depletion of cells labeled with CD235a (Glycophorin A) MicroBeads and CD45 MicroBeads.

Furthermore, CD45 MicroBeads have been used in xenogeneic engraftment studies for the isolation of CD45

⁺

leukocytes.

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Product data and images

Figure 1

Separation of human leukocyte-enriched buffy coat using CD45 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells are stained with CD45-FITC.
Peripheral blood before separation
CD45
^–
cells
CD45
⁺
cells

Figure 2

Enrichment of tumor cells from a mixture of PBMCs and cells of the breast cancer cell line SK-BR3 using CD45 MicroBeads.

(A) Cell mixture before separation (7×106 PBMCs were spiked with 20,000 tumor cells)

(B) Negative fraction with enriched breast cancer cells

A):	B):
Cell mixture before separation (7×10 ⁶ PBMCs were spiked with 20,000 tumor cells)

Figure 3
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Figure 3

A cytokeratin-positive cell was enriched from bone marrow of a patient with renal carcinoma by depletion of leukocytes using CD45 MicroBeads. Cells were stained using a conventional APAAP method followed by staining with hematoxylin. (Courtesy of Dr. Bilkenroth, Dept. Pathol. and Dr. Meye, Dept. Urol., Univ. Halle, Germany.)

A cytokeratin-positive cell was enriched from bone marrow of a patient with renal carcinoma by depletion of leukocytes using CD45 MicroBeads. Cells were stained using a conventional APAAP method followed by staining with hematoxylin. (Courtesy of Dr. Bilkenroth, Dept. Pathol. and Dr. Meye, Dept. Urol., Univ. Halle, Germany.)

Scientific resources

Selected references
1. Meye, A. et al. (2002) Isolation and enrichment of urologic tumor cells in blood samples by a semi-automated CD45 depletion autoMACS protocol. Int. J. Oncol. 21: 521-530
2. Jiang, Y. et al. (2002) Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 418: 41-49
Brochures and posters

MACS Cell Pre-Enrichment for flow sorting

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130-045-801