Name: TCRγ/δ+ T Cell Isolation Kit, mouse
Availability: InStock

Product overview

The TCRγ/δ

⁺

T Cell Isolation Kit has been developed for the isolation of mouse TCRγ/δ

⁺

T cells from single-cell suspensions of spleen, lymph nodes, or thymus.

Background information

T cells bearing γ/δ TCRs are a phenotypically and functionally conserved lineage of T cells which often constitutes the major T cell population in epithelial-rich tissues, e.g. intestine, skin, lung, and reproductive tract. In blood and lymphoid tissues they are found at low frequency. TCRγ/δ

⁺

T cells have been shown to be involved in immune surveillance against tumors, wound healing, as well as pro- and anti-inflammatory responses against pathogens.

The TCRγ/δ

⁺

T Cell Isolation Kit contains CD11b and CD45R (B220) MicroBeads for depletion of CD11b

⁺

and CD45R (B220)

⁺

cells, as well as Anti-TCRγ/δ-Biotin and Anti-Biotin MicroBeads for positive selection of TCRγ/δ

⁺

T cells.

Downstream applications

TCRγ/δ

⁺

T cells isolated by using the TCRγ/δ

⁺

T Cell Isolation Kit are suitable for phenotypic and functional analysis, adoptive transfer, and coculture experiments.

Columns

For the first magnetic separation (depletion): LD or autoMACS

^®

Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

Product data and images

Figure 1

TCRγ/δ

⁺

T cells were isolated from single cell suspensions of mouse spleen (A-C), lymph node (D), and thymus (E) by using the TCRγ/δ

⁺

T Cell Isolation Kit, an LD and two MS Columns, a MidiMACS™ and a MiniMACS™ Separator.

Mouse spleen cells before separation	Pre-enriched splenic TCRγ/δ ⁺ T cells after depletion of CD11b ⁺ and CD45R (B220) ⁺ cells

TCRγ/δ ⁺ T cells isolated from spleen	TCRγ/δ ⁺ T cells isolated from peripheral lymph nodes

TCRγ/δ ⁺ T cells isolated from thymus