Naive CD8a+ T Cell Isolation Kit, mouse

Naive CD8a
+
T Cell Isolation Kit
, mouse

The Naive CD8a
+
T Cell Isolation Kit was developed for the isolation of untouched naive CD8a
+
T cells from mouse spleen and lymph node cells.

Detailed separation procedure

For magnetic depletion of memory CD8
+
T cells and non-CD8
+
T cells, single cell suspension is incubated with a cocktail of biotinylated CD45R, CD4, CD11b, CD11c, CD19, CD44, CD49b, CD105, Ter-119, MHC II, and anti-TCRγ/δ antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion.

Downstream applications

Naive CD8a
+
T cells can be used for further
in vitro
analysis of T cell differentiation or adoptive transfer experiments.

Columns

MS, LS, or autoMACS
®
Columns.
  • Selected references

    1. Hinrichs, C. S. et al. (2009) Adoptively transferred effector cells derived from naive rather than central memory CD8+ T cells mediate superior antitumor immunity. Proc. Natl. Acad. Sci. U.S.A. 106(41): 17469-17474
    2. Kamimura, D. and Bevan, M. J. (2007)
      Naive CD8
      +
      T cells differentiate into protective memory-like cells after IL-2 anti IL-2 complex treatment
      in vivo
      .
      J. Exp. Med. 204(8): 1803-1812
Product options: 1
for 1×
10
9
total cells
USD 685.00

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