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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
T cells and CD19+
B cells were isolated from 5 mL of human EDTA-anticoagulated whole blood using the MACSprep™ HLA B/T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress®
Separator. The isolated cells were fluorescently stained with CD45-VioBlue, CD3-FITC, CD19-APC, and CD56-PE and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.
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