CD45RA MicroBeads, human

CD45RA MicroBeads, human

CD45RA MicroBeads were developed for positive selection or depletion of lymphocyte subsets from human PBMCs.

Background information

CD45RA is expressed on naive CD4
+
and CD8
+
T cells as well as on CD8
+
effector T cells. CD45RA is also present on subsets of B and NK cells as well as on plasmacytoid dendritic cells. The CD45RA antibody recognizes the 220 kDa isoform of the leukocyte common antigen (LCA).
CD45RA MicroBeads are used for the positive selection or depletion of CD45RA
+
cells. For isolation of CD45RA
+
T cell subsets, the CD45RA MicroBeads can be combined with MACS
®
Cell Isolation Kits
6,7
or CD4 and CD8 MultiSort Kits
1,2
. The CD45RA MicroBeads can also be used to isolate CD45RO
+
cells from pre-sorted CD4
+
or CD8
+
T cells by depletion of CD45RA
+
cells.
3,4,5,7

Downstream applications

Cells isolated with CD45RA MicroBeads can be used for various studies, such as cytokine expression or receptor signaling.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
  • Selected references

    1. Wolthers, K. C. et al. (1999) Normal T-cell telomerase activity and upregulation in human immunodeficiency virus-1 infection. Blood 93: 1011-1019
    2. Jonuleit, H. et al. (2000)
      Induction of interleukin 10-producing, nonproliferating CD4
      +
      T cells with regulatory properties by repetitive stimulation with allogeneic immature human dendritic cells.
      J. Exp. Med. 192: 1213-1222
    3. Mattila, P. S. et al. (2000)
      Adenoids provide a microenvironment for the generation of CD4
      +
      , CD45RO
      +
      , L-selectin
      , CXCR4
      +
      , CCR5
      +
      T lymphocytes, a lymphocyte phenotype found in the middle ear effusion.
      Int. Immunol. 12: 1235-1243
    4. Matsushima, T. et al. (2001) Receptor binding cancer antigen expressed on SiSo cells, a novel regulator of apoptosis of erythroid progenitor cells. Blood 98: 313-321
    5. Akdis, M. et al. (1999)
      Skin homing (cutaneous lymphocyte-associated antigen-positive) CD8
      +
      T cells respond to superantigen and contribute to eosinophilia and IgE production in atopic dermatitis.
      J. Immunol. 163: 466-475
    6. Hafsi, N. et al. (2004)
      Human dendritic cells respond to
      Helicobacter pylori
      , promoting NK cell and Tʜ1-effector responses
      in vitro
      .
      J. Immunol. 173: 1249-1257
    7. Verhasselt et al. (2004) Induction of FOXP3-expressing regulatory CD4pos T cells by human mature autologous dendritic cells. Eur. J. Immunol. 34: 762-772
  • Customer reports and application notes

Product options: 1
for 1×
10
9
total cells
USD 665.00

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