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regulatory T cells were isolated from human PBMCs by using the CD4+
Regulatory T Cell Isolation Kit, an LD, and two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. The isolated cells were fluorescently stained with CD4, CD25, and either CD45RA or Anti-FoxP3 antibodies. Dot plots show data gated on viable lymphocytes (C) or viable CD4+
lymphocytes (A, B).
Viable CD4+ lymphocytes
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