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regulatory T cells were isolated from human PBMCs using the CD25+
Regulatory T Cell Isolation Kit, an LD Column, two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. Cells were fluorescently stained with CD4-FITC, CD25-PE, CD49d-Biotin and Anti-Biotin-APC, or CD4-FITC, CD25-PE, and Anti-FoxP3-APC and analyzed by flow cytometry using the MACSQuant® Analyzer.
PBMCs before separation
Pre-enriched CD25+ CD49d- Treg cells
Isolated CD25+ CD49d- Treg cells
Gated on CD4+
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