Name: CD137 MicroBead Kit, human
Availability: InStock

Product overview

The CD137 MicroBead Kit was developed for the positive selection of human CD137

⁺

cells from PBMCs.

Background information

CD137-PE recognizes the CD137 (4-1BB) antigen, a 30 kDa glycoprotein of the tumor necrosis factor (TNF) receptor superfamily. CD137 is mainly expressed on activated CD4

⁺

and CD8

⁺

T cells, activated B cells, and on natural killer cells, but can also be found on resting monocytes and dendritic cells. As a costimulatory molecule CD137 is involved in the activation and survival of CD4

⁺

or CD8

⁺

T cells, and NK cells.

Recently, CD137 has been described to be a suitable marker for antigen-specific activation of human CD8

⁺

T cells, as CD137 is not expressed on resting CD8

⁺

T cells and its expression is reliably induced after 24 hours of stimulation.

CD137

⁺

cells are indirectly magnetically labeled with CD137-PE and Anti-PE MicroBeads. For the isolation of activated antigen-specific CD8

⁺

T cells, the CD137 MicroBead Kit can be combined with the CD8

⁺

T Cell Isolation Kit.

Downstream applications

In a previous study, CD137-expressing T cells were targeted for selective depletion of alloreactive T cells from antileukemic and antitumor donor T cell lines.

The CD137 MicroBead Kit was developed for positive selection of CD137

⁺

cells for phenotypical and functional characterization of activated CD137

⁺

T cells.

Columns

MS, LS, or autoMACS

^®

Columns.

Product data and images

Figure 1

PBMCs of a CMV

⁺

donor were stimulated overnight with PepTivator™ CMV pp65 or left untreated. The responding CD137

⁺

T cells were separated using the CD137 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were additionally stained with CD8-FITC.

Stimulated sample

Unseparated fraction	After separation

Unstimulated control

Unseparated fraction	After separation

Scientific resources

Selected references
1. Schmied, S. et al. (2015)
  Analysis of the functional WT1-specific T-cell repertoire in healthy donors reveals a discrepancy between CD4
  ⁺
  and CD8
  ⁺
  memory formation.
  Immunology 145(4): 558-569
2. Bacher, P. et al. (2014) Antigen-specific expansion of human regulatory T cells as a major tolerance mechanism against mucosal fungi. Mucosal Immunol 7(4): 916-928
3. Wolfl, M. et al. (2007)
  Activation-induced expression of CD137 permits detection, isolation, and expansion of the full repertoire of CD8
  ⁺
  T cells responding to antigen without requiring knowledge of epitope specificities.
  Blood 110: 201-210
4. Wehler, T. C. et al. (2007) Targeting the activation-induced antigen CD137 can selectively deplete alloreactive T cells from antileukemic and antitumor donor T-cell lines. Blood 109: 365-373
Customer reports and application notes

Detection, enrichment, and direct phenotypic and functional characterization of antigen-specific naive and memory T cell subsets. Bacher, P. & Scheffold, A. (2015)

Product options: 1

130-093-476