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Splenocytes from a C57BL/6 mouse were isolated by using the NK1.1+
iNKT Cell Isolation Kit, an LD and two MS Columns, a MidiMACS™ and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-NK1.1-APC, CD3ε-VioBlue, and α-Gal-Cer/CD1d Tetramer-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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