CD57 MicroBeads, human

CD57 MicroBeads, human

CD57 MicroBeads were developed for the positive selection or depletion of CD57
+
cells from human PBMCs, body fluids, or single-cell suspensions from tissue (e.g. lymphoid tissues).

Background information

The CD57 antigen, also known as HNK-1 or Leu-7, is an oligosaccharide antigenic moiety bound to surface proteins of certain cell types. CD57 is present on 15–20% of PBMCs, including subsets of NK cells and T cells and some neural cell types.
1,2
CD57 has been shown to be expressed on late-stage CD8
+
effector cells
3
and terminally differentiated NK cells.
The frequency of CD57
+
T lymphocytes is raised in a variety of diseases, for example, in rheumatoid arthritis
4
. Its expression is also increased on proliferation-incompentent or chronically activated CD8
+
T cells in persistent viral infections, such as HIV.
5

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
  • Selected references

    1. Mechtersheimer, G. et al. (1991) Expression of the natural killer cell-associated antigens CD56 and CD57 in human neural and striated muscle cells and in their tumors. Cancer Res. 51: 1300-1307
    2. Ong, E. et al. (2002)
      Biosynthesis of HNK-1 glycans on O-linked oligosaccharides attached to the neural cell adhesion molecule (NCAM): the requirement for core 2 β1,6-
      N
      -acetylglucosaminyltransferase and the muscle-specific domain in NCAM.
      J. Biol. Chem. 277: 18182-18190
    3. Brenchley, J. M. et al. (2003)
      Expression of CD57 defines replicative senescence and antigen-induced apoptotic death of CD8
      +
      T cells.
      Blood 101: 2711-2720
    4. Dupuy d'Angeac, A. et al. (1993)
      Increased percentage of CD3
      +
      , CD57
      +
      lymphocytes in patients with rheumatoid arthritis. Correlation with duration of disease.
      Arthritis Rheum. 36: 608-612
    5. Palmer, B. E. et al. (2005)
      Functional and phenotypic characterization of CD57
      +
      CD4
      +
      T cells and their association with HIV-1-induced T cell dysfunction.
      J. Immunol. 175: 8415-8423
  • Brochures and posters

Product options: 1
for 1×
10
9
total cells
USD 660.00