As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Neuronal cells were isolated from P2 and from 9 weeks old CD-1 ® mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Neonatal cells |
Before separation | Isolated neuronal cells | Non-neuronal cells |
 |  |  |
 |  |  |
 |  |  |
Adult cells | ||
Before separation | Isolated neuronal cells | Non-neuronal cells |
 |  |  |
 |  |  |
 |  |  |
Copyright © 2019 Miltenyi Biotec and/or its affiliates. All rights reserved.
