CD115 MicroBead Kit, mouse

CD115 MicroBead Kit, mouse

The CD115 MicroBead Kit has been developed for positive selection of CD115
mouse cells from lymphoid tissues.

Background information

CD115 is expressed on monocytes, macrophages, osteoclasts, as well as on common dendritic cell precursors and macrophage/dendritic cell precursors. CD115 has been proposed as a marker for the standardized identification and analysis of mouse monocytes.

Detailed separation procedure

In order to avoid non-specific binding of the antibody, the single-cell suspension is pre-incubated with FcR Blocking Reagent. Target cells are then labeled with a biotinylated CD115 antibody. Subsequently, CD115
cells are magnetically labeled with Anti-Biotin MicroBeads. After the separation over a MACS Column, the CD115
cells are eluted as the positive fraction.

Downstream applications

Isolated mouse CD115
cells , e.g. from bone marrow, can be used for
in vitro
analysis, differentiation studies, co-culture, or adoptive transfer experiments.


MS, LS, or autoMACS
  • Selected references

    1. Breslin W. L. et al. (2013) Mouse blood monocytes: Standardizing their identification and analysis using CD115. J. Immunol. Methods 390(1-2): 1-8
Product options: 1
for 1×
total cells
USD 550.00

Related products

5 products available | view all