Indirect CD133 MicroBead Kit, human

Indirect CD133 MicroBead Kit, human

The Indirect CD133 MicroBead Kit was developed for positive selection or depletion of CD133
+
cells, using a two-step procedure involving indirect magnetic labeling.
  • Hematopoietic stem cells can be isolated from peripheral blood, cord blood, bone marrow, or leukapheresis product.
  • Neural progenitor cells can be isolated from single-cell suspensions from primary neural tissues or cell lines.
  • ES and iPS cell–derived neural, endothelial, or hematopoietic progenitors can be isolated from differentiated ES or iPS cell cultures1 .
  • Cancer stem cells can be isolated from single-cell suspensions from primary tumor tissue or cell lines.

Background information

CD133, formerly known as AC133, recognizes epitope 1 of the CD133 antigen.
2,3
It is a marker that is frequently found on multipotent progenitor cells, including immature hematopoietic stem and progenitor cells. In the hematopoietic system, CD133 is expressed on a small portion of CD34
cells
4
as well as on a subset of CD34
bright
stem and progenitor cells in human fetal liver, bone marrow, cord blood, and peripheral blood
5
. CD133 has also been found to be expressed on circulating endothelial progenitor cells;
6,7
fetal neural stem cells;
8,9
other tissue-specific stem cells, such as renal
10
, prostate
11
, and corneal
12
stem cells; cancer stem cells from tumor tissues; as well as ES and iPS cell-derived cells.
There is a growing interest in CD133 antigen expressing stem cells from normal blood or bone marrow in the field of regenerative medicine, for example bone marrow-derived CD133
+
stem cells in cardiovascular
13-17
, liver, or peripheral artery diseases
18-20
.
The CD133 antibody included in the kit recognizes epitope CD133/1. For quality control staining of CD133-separated cells, the use of CD133/2 (293C3)-PE or -APC is recommended.

Downstream applications

Isolated from hematopoietic sources, CD133
+
cells can become adherent and are reported to become CD133-negative during culture.
21,22
These adherent cells can then in turn give rise to nonadherent CD133
+
cells that are able to differentiate to both hematopoietic and nonhematopoietic cell types.
23
CD133
+
cells have shown a capacity for tissue differentiation, including to neural lineages
24
. CD133
+
isolated from fetal liver
25
, umbilical cord blood
26
, bone marrow
27
, mobilized blood
28
, and skin
29
are capable of in vitro differentiation to neuronal cells as well as to astrocytes,
25,26,28
oligodendrocytes,
26,28
and glial cells.
26
CD133
+
cells isolated from human fetal brain
8,9,30-32
were able to form self-renewing neurospheres in vitro, and to differentiate into neurons
8,32
and glia
19,23
. When injected into mice, human CD133
+
cells differentiated into fully integrated neurones and glial cells
9,30
as well as astrocytes and endothelial cells
29
. The CD34
+
CD133
+
cell population, which includes CD34
+
CD38
cells, was shown to be capable of repopulating NOD/SCID mice
33
.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
  • Selected references

    1. Galic, Z. et al. (2006) T lineage differentiation from human embryonic stem cells. Proc. Natl. Acad. Sci. U.S.A. 103: 11742-11747
    2. Yin, A. H. et al. (1997) AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood 90: 5002-5012
    3. Piechaczek, C. (2001) CD133. J. Biol. Regul. Homeost. Agents 15: 101-102
    4. Gallacher, L. et al. (2000)
      Isolation and characterization of human CD34
      Lin
      and CD34
      +
      Lin
      hematopoietic stem cells using cell surface markers AC133 and CD7.
      Blood 95(ARVO Annual Meeting Abstract): 2813-2820
    5. Bühring, H. J. et al. (1999) Expression of novel surface antigens on early hematopoietic cells. Ann. N. Y. Acad. Sci. 872: 25-39
    6. Gehling, U. M. et al. (2000)
      In vitro
      differentiation of endothelial cells from AC133-positive progenitor cells.
      Blood 95: 3106-3112
    7. Peichev, M. et al. (2000)
      Expression of VEGFR-2 and AC133 by circulating human CD34
      +
      cells identifies a population of functional endothelial precursors.
      Blood 95: 952-958
    8. Uchida, N. et al. (2000) Direct isolation of human central nervous system stem cells. Proc. Natl. Acad. Sci. U.S.A. 97: 14720-14725
    9. Cummings, B. J. et al. (2005) Human neural stem cells differentiate and promote locometer recovery in spinal cord-injured mice. Proc. Natl. Acad. Sci. U.S.A. 102: 14069-14074
    10. Bussolati, B. et al. (2005) Isolation of renal progenitor cells from adult human kidney. Am. J. Pathol. 166: 545-555
    11. Richardson, G. et al. (2004) CD133, a novel marker for human prostatic epithelial stem cells. J. Cell. Sci. 117: 3539-3545
    12. Thill, M. et al. (2004)
      Identification of a population of CD133
      +
      precursor cells in the stroma of human cornea.
      Invest. Ophthalmol. Vis. Sci. 45: 3519
    13. Stamm, C. et al. (2003) Autologous bone-marrow stem-cell transplantation for myocardial regeneration. Lancet 4, 361(9351): 45-46
    14. Stamm, C. et al. (2007)
      Intramyocardial delivery of CD133
      +
      bone marrow cells and coronary artery bypass grafting for chronic ischemic heart disease: safety and efficacy studies.
      J. Thorac. Cardiovasc. Surg. 133: 717-725
    15. Klein (2007) Eur. Cardiovasc. Dis. 1: 123-125
    16. Klein, H. M. et al. (2007)
      Intramyocardial implantation of CD133
      +
      stem cells improved cardiac function without bypass surgery.
      Heart Surg. Forum 10: E66-69
    17. Bartunek et al. (2005) Circulation 30: 178-183
    18. am Esch, J. S. 2nd et al. (2005)
      Portal application of autologous CD133
      +
      bone marrow cells to the liver: a novel concept to support hepatic regeneration.
      Stem Cells 23(4): 463-470
    19. Fürst, G. et al. (2007)
      Portal vein embolization and autologous CD133
      +
      bone marrow stem cells for liver regeneration: initial experience.
      J. Immunother. 243(1): 171-179
    20. Cañizo et al. (2007)
      Peripheral endothelial progenitor cells (CD133
      +
      ) for therapeutic vasculogenesis in a patient with critical limb ischemia. One year follow-up.
      Cytotherapy 9(1): 99-102
    21. Kuçi et al. (2003)
      Identification of a novel class of human adherent CD34
      stem cells that give rise to SCID-repopulating cells.
      Blood 101: 869-876
    22. Kuçi et al. (2008)
      Efficient
      in vitro
      generation of adult multipotent cells from mobilized peripheral blood CD133
      +
      cells.
      Cell Prolif. 41: 12-27
    23. Kuçi et al. (2003) MACS&more 7(1): 6-8
    24. Kuçi et al. (2004) 2nd Intl. Meeting, Stem Cell Network, North-Rhine Westphalia. : abstract
    25. Hao, H. N. et al. (2003)
      Fetal human hematopoietic stem cells can differentiate sequentially into neural stem cells and then astrocytes
      in vitro
      .
      J. Hematother. Stem Cell Res. 12: 23-32
    26. Jang, Y. K. et al. (2004) Retinoic acid-mediated induction of neurons and glial cells from human umbilical cord-derived hematopoietic stem cells. J. Neurosci. Res. 75: 573-584
    27. Padovan et al. (2003) Expression of neuronal markers in differentiated marrow stromal cells and CD133+ stem-like cells. Cell Transplant 12: 839-848
    28. Piechaczek, C. et al. (2002)
      Differentiation of adult CD133
      +
      cells isolated from peripheral blood into cells with a neural phenotype.
      Stem cell research (customer reports published by Miltenyi Biotec) : 2-3
    29. Belicchi, M. et al. (2004) Human skin-derived stem cells migrate throughout forebrain and differentiate into astrocytes after injection into adult mouse brain. J. Neurosci. Res. 77: 475-486
    30. Tamaki, S. et al. (2002) Engraftment of sorted/expanded human central nervous system stem cells from fetal brain. J. Neurol. Res. 69: 976-986
    31. Kelly, S. et al. (2004) Transplanted human fetal neural stem cells survive, migrate, and differentiate in ischemic rat cerebral cortex. Proc. Natl. Acad. Sci. U.S.A. 101: 11839-11844
    32. Yu, S. et al. (2004)
      Isolation and characterization of the CD133
      +
      precursors from the ventricular zone of human fetal brain by magnetic affinity cell sorting.
      Biotechnol. Lett. 26: 1131-1136
    33. de Wynter, E. A. et al. (1998)
      CD34
      +
      AC133
      +
      cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors.
      Stem Cells 16: 387-396
Product options: 1
for 2×
10
9
total cells
USD 715.00

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