CD34 MultiSort Kit, human

CD34 MultiSort Kit, human

The CD34 MultiSort Kit has been developed for the positive selection of CD34
+
subpopulations by sequential sorting. CD34
+
hematopoietic stem and progenitor cells can be isolated from
  • peripheral blood,
  • bone marrow aspirate,
  • leukapheresis product,
  • cord blood,
  • differentiated ES and iPS cell cultures1 .

Detailed separation procedure

Using the CD34 MultiSort Kit, in the first step CD34
+
cells are positively selected after labeling with the CD34 MultiSort MicroBeads. Subsequently, the MultiSort MicroBeads are enzymatically released from the CD34 antibody. The isolated CD34
+
cells can be magnetically labeled and sorted according to a second antigen. The kit contains CD34 MultiSort MicroBeads, MultiSort Release Reagent, and MultiSort Stop Reagent.
Control staining after separation requires a monoclonal CD34 antibody other than QBEND/10, e.g. clone AC136

Downstream applications

The CD34 MultiSort Kit is used to isolate specific subsets of CD34
+
hematopoietic stem and progenitor cells. CD34-selected cells can be further sorted according to their expression of, e.g., HLA-DR, CD43, CD45RA, CD61, CD71, CD90 (Thy1), or CD133.

Columns

MS, LS, XS, or autoMACS
®
Columns.

  • Figure 1

    CD34
    +
    HLA-DR– cells isolated from human PBMCs using the CD34 MultiSort Kit. Positive selection of CD34
    +
    cells from PBMCs was followed by the depletion of CD34
    +
    HLA-DR
    +
    cells using HLA-DR-FITC and Anti-FITC MicroBeads resulting in a population of CD34
    +
    HLA-DR
    –
    cells (red).
    A:
    B:
    PBMCs before separation
    CD34
    +
    cells
    C:
    CD34
    +
    HLA-DR
    -
    cells

  • Figure 2

    View details

    Figure 2

    Step-by-step CD34 MultiSort separation procedure.
    Step-by-step CD34 MultiSort separation procedure.

  • Figure 3

    View details

    Figure 3

    CD34
    +
    lin
    -
    cells isolated from peripheral blood using CD34 MultiSort Kit, May-Grünwald-Giemsa stained. (Courtesy of Dr. Ziegler, Tübingen, Germany.)
    CD34
    +
    lin
    -
    cells isolated from peripheral blood using CD34 MultiSort Kit, May-Grünwald-Giemsa stained. (Courtesy of Dr. Ziegler, Tübingen, Germany.)
Product options: 1
130-056-701

CD34 MultiSort Kit, human

for 2×
10
9
total cells
USD 755.00

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