CD117 MicroBeads, mouse

CD117 MicroBeads, mouse

CD117 MicroBeads have been developed for the positive selection of hematopoietic stem and progenitor cells from mouse bone marrow . In addition, CD117 MicroBeads have been successfully used for the isolation of a variety of tissue stem cells, e.g. differentiated embryonic stem cell cultures
1
, or amniotic fluid-derived stem cells
2
.

Background information

CD117 (c-kit) is a receptor tyrosine kinase expressed on the majority of hematopoietic stem and progenitor cells, on some tissue stem cells as well as mature mast cells. Stimulation of CD117 by its ligand, the cytokine stem cell factor (SCF), is important for stem cell survival and maintance.

Downstream applications

  • Further flow cytometric analysis or sorting of isolated CD117+ cells, e.g. into LSK populations3 .
  • Transplantation of CD117+ bone marrow cells, e.g. after myocardial infarction for differentiation into smooth muscle cells, myocytes, and endothelial cells4 .

Columns

MS, LS, XS, or autoMACS
®
Columns.
  • Selected references

    1. Verda, L. et al. (2008) Hematopoietic mixed chimerism derived from allogeneic embryonic stem cells prevents autoimmune diabetes mellitus in NOD mice. Stem Cells 26: 381-386
    2. De Coppi, P. et al. (2007) Isolation of amniotic stem cell lines with potential for therapy. Nat. Biotechnol. 25: 100-106
    3. Shin, J.Y. et al. (2014) High c-Kit expression identifies hematopoietic stem cells with impaired self-renewal and megakaryocytic bias J. Exp. Med. 211(2): 217-231
    4. Orlic, D. et al. (2001) Mobilized bone marrow cells repair the infarcted heart, improving function and survival. Proc. Natl. Acad. Sci. U.S.A. 98: 10344-10349
Product options: 2
for 1×
10
9
total cells
USD 580.00
for 1×
10
9
total cells
USD 575.00

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