Anti-Sca-1 MicroBead Kit (VioBright™ FITC), mouse

Anti-Sca-1 MicroBead Kit (VioBright™ FITC), mouse

The Anti-Sca-1 MicroBead Kit (VioBright™ FITC), mouse has been developed for the positive selection of hematopoietic stem and progenitor cells from mouse bone marrow. Sca-1
+
stem cells have been isolated from peripheral blood
1
, fetal
2
, and adult liver
3
, as well as heart
4
and prostate tissue
5
. The kit can be used either alone or in combination with the Lineage Cell Depletion Kit, mouse for enrichment of KSL cells as well as in combination with the CD105 MultiSort Kit (PE), mouse for the enrichment of long-term repopulating hematopoietic stem cells (LT-HSCs).

Background information

Stem cell antigen-1 (Sca-1) is one of the defining markers for KSL cells (c-
k
it
+ S
ca-1
L
in
), the population that comprises the stem cell compartment in mouse bone marrow. Sca-1 expression in combination with CD105 can be further used to define long-term repopulating hematopoietic stem cells (LTR-HSCs).
6,7
The Sca-1 protein is encoded by one of the two Ly-6A/E alleles expressed in different mouse strains (Ly-6E.1: e.g. BALB/c, C3H, NZB; Ly-6A.2: e.g. C57BL/6, SJL, 129, AKR). The anti-Sca-1 monoclonal antibody included in the kit recognizes both, Ly-6E.1 and Ly-6A.2.

Downstream applications

Bone marrow-derived Sca-1
+
cells have been used for nonhematopoietic differentiation into hepatocytes
in vivo8
and neural cells
in vitro9
. ES cell-derived SCA-1
+
cells could be differentiated into endothelial cells with high purity and yield.
10
Furthermore, smooth muscle cells have been generated from ESCs via isolation of Sca-1
+
cells.
11

Columns

MS, LS, XS, or autoMACS
®
Columns.
  • Selected references

    1. Yamada, M. et al. (2004) Bone marrow-derived progenitor cells are important for lung repair after lipopolysaccharide-induced lung injury. J. Immunol. 172: 1266-1272
    2. Burger, P. E. et al. (2005) Sca-1 expression identifies stem cells in the proximal region of prostatic ducts with high capacity to reconstitute prostatic tissue. Proc. Natl. Acad. Sci. U.S.A. 102: 7180-7185
    3. Chen, C. Z. et al. (2002) Identification of endoglin as a functional marker that defines long-term repopulating hematopoietic stem cells. Proc. Natl. Acad. Sci. U.S.A. 99: 15468-15473
  • Special protocols

Product options: 1
for 1×
10
9
total cells
USD 665.00

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