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cells were isolated from mouse spleen cells by using the Anti-Ly-6G MicroBead Kit, two MS Columns and a MiniMACS™ Separator. The cells are fluorescently stained with Anti-Gr-1-PE (clone RB6-8C5) and CD11b-FITC. Cell debris and dead cells were excluded from the analysis based on scatter signals and propodium iodide fluorescence.
Spleen cells before separation
Spleen cells depleted of Ly-6G+
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