Name: CD31 MicroBeads, mouse
Availability: InStock

Product overview

The CD31 MicroBeads, mouse, have been developed for the positive selection or depletion of CD31

⁺

endothelial cells from single-cell suspensions of mouse tissues.

Background information

The CD31 antibody reacts with the cell surface protein CD31. The murine CD31 antigen is also known as PECAM-1. CD31 is a single-pass type I membrane protein containing six Ig-like C2-type domains and functions as cell adhesion molecule

. It is present on mature endothelial cells as well as on most leukocyte subtypes and platelets

where the expression level can vary. Besides its function in cell adhesion, the protein is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions

Downstream applications

Mouse endothelial cells isolated with CD31 MicroBeads are immediately ready to be used for phenotype analysis, coculture experiments, or functional studies, for example, in tumor vascularization research.

Columns

For positive selection: MS, LS, or autoMACS

^®

Columns. For depletion: LD or autoMACS Columns.

Product data and images

Figure 1

Mouse CD31

⁺

endothelial cells (bEnd.3 cells) were isolated from a mixture of U937 and bEnd.3 cells using CD31 MicroBeads, an MS Column, and an OctoMACS™ Separator. Cells were fluorescently stained with CD31-PE and analyzed using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Unseparated fraction	CD31 ^– cells

CD31 ⁺ cells

Scientific resources

Selected references
1. Woodfin, A. et al. (2007) PECAM-1: a multi-functional molecule in inflammation and vascular biology. Arterioscler. Thromb. Vasc. Biol. 27(12): 2514-2523
2. Xie, Y. and Muller, W. A. (1993) Molecular cloning and adhesive properties of murine platelet/endothelial cell adhesion molecule 1. Proc. Natl. Acad. Sci. U.S.A. 90(12): 5569-5573
3. van Buul, J. D. and Hordijk, P. L. (2008) Endothelial signaling by Ig-like cell adhesion molecules. Transfus. Clin. Biol. 15(1-2): 3-6
Special protocols

Isolation of endothelial cells from mouse neonatal brain

Product options: 1

130-097-418