Name: Myeloid Dendritic Cell Isolation Kit, human
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Product overview

The Myeloid Dendritic Cell Isolation Kit has been developed for the concurrent isolation of untouched CD1c (BDCA-1)

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and CD141 (BDCA-3)

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myeloid dendritic cells from human PBMCs.

Detailed separation procedure

Non-MDCs are labeled with a cocktail of biotinylated antibodies and Anti-Biotin MicroBeads for depletion over a MACS Column. Unlabeled MDCs pass through the column.

Downstream applications

MDCs isolated with the Myeloid Dendritic Cell Isolation Kit are used when direct labeling of surface molecules is undesired.

Columns

LD or autoMACS

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Columns.

Figure 1

MDCs were isolated from human PBMCs using the Myeloid Dendritic Cell Isolation Kit. Cells were fluorescently stained with CD141 (BDCA- 3)-FITC, CD20-PE, CD14-VioBlue, and CD1c (BDCA-1)-APC and analyzed by flow cytometry. Exclusion of CD1c

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CD20

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B cells and CD14

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monocytes was performed. Shown are CD1c (BDCA-1) and CD141 (BDCA-3) stainings of total cells before and after separation.

Unseparated fraction	After separation

Scientific resources

Selected references
1. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046

Product options: 1

130-094-487