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Human PBMCs depleted of CD14+
cells were stimulated for 4 hours with LPS (10 ng/ml) and for 2 hours with R-848 (2.5 μg/ml), or left untreated. The stimulated and unstimulated cells were stained and isolated according to the secretion of IL-12 using the IL-12 Secretion Assay - Cell Enrichment and Detection Kit. Additionally, cells were stained with CD11c-APC.
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