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cells were isolated from mouse bone marrow (A) or mouse fetal liver (B) using CD93 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD93-APC and Labeling Check Reagent-FITC and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
A) Bone marrow
B) Fetal liver