CD19 MultiSort Kit, human

CD19 MultiSort Kit, human

The CD19 MultiSort Kit was developed for the fast and efficient isolation of CD19
+
B cell subsets from human PBMCs, bone marrow, or lymphoid tissues. The CD19 MultiSort Kit is a complete system for sequential positive selections.

Detailed separation procedure

Lineage B cells are isolated with CD19 MultiSort MicroBeads and subsequently the magnetic particles are enzymatically released. The selected CD19
+
B cells can then be magnetically labeled and sorted according to a second antigen. The kit contains CD19 MultiSort MicroBeads, the MultiSort Release Reagent, and the MultiSort Stop Reagent.

Applications

The CD19 MultiSort Kit can be used for positive selection or depletion of B cell populations that express distinct developmental markers such as CD5 (e.g. B-1 cells), CD27 (e.g. memory B cells)
1
, or IgD (e.g. naive B cells)
2
. It can be further used for the isolation of activated B cells according to activation markers, e.g., CD25, CD30, CD38, or CD69. For selection of specific B cell subsets please refer also to the subset kits available.

Columns

MS, LS, XS, or autoMACS
®
Columns.
  • Selected references

    1. De Milito, A. et al. (2004) Mechanisms of hypergammaglobulinemia and impaired antigen-specific humoral immunity in HIV-1 infection. Blood 103: 2180-2186
    2. Al-Darmaki et al. (2004) Delineation of the role of platelet-activating factor in the immunoglobulin G2 antibody response. Clin. Diagn. Lab. Immunol. 11: 720-728
Product options: 1
for 1×
10
9
total cells
USD 765.00

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