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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
A single-cell suspension from mouse spleen was prepared using the gentleMACS™ Dissociator. CD19+
B cells were isolated from this single-cell suspension using the CD19 MicroBeads, and autoMACS®
Pro Separator. Cells were fluorescently stained with REAfinity™ Antibodies CD45R (B220)-APC-Vio®
770 (clone REA755), mouse, and CD19-PE-Vio770, mouse (clone REA749) and analyzed by flow cytometry using the MACSQuant®
Analyzer. Viable leukocytes were gated for analysis based on scatter signals and 7-AAD Staining Solution fluorescence.