CD19 MicroBeads, human

CD19 MicroBeads, human

CD19 MicroBeads were developed for positive selection or depletion of CD19
+
B cells from different cell sources such as human PBMCs, bone marrow, lymphoid tissues, or cell cultures.

Background information

As a B cell lineage marker, the CD19 antigen is expressed from the early pro-B cell stage to the B cell lymphoblast stage, but the expression is down-regulated upon B cell maturation to plasma cells. The CD19 antigen is further expressed on most malignant B cells and on a subset of follicular dendritic cells.

Downstream applications

Isolated CD19
+
B cells were used for studies on immunoglobulin production, such as IgE synthesis
1, 2
and secretion
3
, chemotaxis assays
4
, or mixed lymphocyte reactions. Positively selected CD19
+
B cells were also used for molecular studies such as gene expression profiling.
5
For the analysis of minimal residual diseases in B cell malignancies, enrichment of B cells increases the sensitivity of detection.
6

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
  • Selected references

    1. Aebischer et al. (1994)
      Neuropeptides are potent modulators of human
      in vitro
      immunoglobulin E synthesis.
      Eur. J. Immunol. 24: 1908-1913
    2. Stämpfli et al. (1994) Inhibition of human IgE synthesis by anti-IgE antibodies requires divalent recognition. Eur. J. Immunol. 24: 2161-2167
    3. Zürcher, A. W. et al. (1995) IgE-producing hybridomas established after B-cell culture in the CD40 system. Immunol. Lett. 46: 49-57
    4. Schratzberger, P. et al. (1997) Differential chemotactic activities of sensory neuropeptides for human peripheral blood mononuclear cells. J. Immunol. 158: 3895-3901
    5. Klein, U. et al. (2001) Gene expression profiling of B cell chronic lymphocytic leukemia reveals a homogeneous phenotype related to memory B cells. J. Exp. Med. 194: 1625-1638
    6. Pugh, R. E. et al. (1998) CD19 selection improves the sensitivity of B cell lymphoma detection. J. Hematother. 7: 159-168
  • Brochures and posters

Product options: 2
for 1×
10
9
total cells
USD 675.00
for 1×
10
9
total cells
USD 670.00

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