Name: B Cell Isolation Kit II, human
Availability: InStock

Product overview

The B Cell Isolation Kit II was developed for the fast and efficient isolation of untouched B cells from human PBMCs.

Detailed separation procedure

Non-B cells (T cells, NK cells, monocytes, dendritic cells, granulocytes, platelets, and erythroid cells) are labeled with a cocktail of biotinylated CD2, CD14, CD16, CD36, CD43, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion. Highly pure B cells are obtained by depletion of magnetically labeled non-B cells.

Downstream applications

Untouched B cells obtained with the B Cell Isolation Kit II can be used for the isolation of specific B cell subsets, including naive and memory B cells.

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Columns

MS, LS, XS, or autoMACS

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Columns.

Product data and images

Figure 1

Untouched B cells were isolated from PBMCs using the B Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator.
Unseparated fraction
Untouched isolated B cells
Figure 2
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Figure 2

Scanning electron micrograph of a B cell isolated with the B Cell Isolation Kit.

(Courtesy of Prof. Groscurth, Zürich, Switzerland).

Scanning electron micrograph of a B cell isolated with the B Cell Isolation Kit.

(Courtesy of Prof. Groscurth, Zürich, Switzerland).
Figure 3
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Figure 3

Isolation of untouched B cells using the B Cell Isolation Kit II.

Isolation of untouched B cells using the B Cell Isolation Kit II.

Scientific resources

Selected references
1. Poeck, H. et al. (2004) Plasmacytoid dendritic cells, antigen, and CpG-C license human B cells for plasma cell differentiation and immunoglobulin production in the absence of T-cell help. Blood 103: 3058-3064
2. Marinkovic, D. et al. (2004) Identification of novel Myc target genes with a potential role in lymphomagenesis. Nucleic Acids Res. 32: 5368-5378
3. Jaimes, M. C. (2004) Maturation and trafficking markers on rotavirus-specific B cells during acute infection and convalescence in children. J. Virol. 78: 10967-10976

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130-091-151