Poly (I:C)

Poly (I:C)

TLR3 is a member of the toll-like-receptor (TLR) family recognizing pathogen-associated molecular patterns.
1
TLR3 recognizes (virally derived) double- or single-stranded RNA leading to activation of immune cells. The natural ligands for TLR3 can be mimicked by synthetic RNAs (oligoribonucleotides; ORNs) like polyinosinic-polycytidylic acid (poly (I:C)), which hybridizes to form RNA double strands in aqueous solutions with ionic strength.

Background information

Poly (I:C) can activate the nuclear transcription factor NF-κB1 as well as stimulate the production of type I interferons (IFNs) leading to cytokine secretion
2,3
. Poly (I:C) is one of the most potent agonists for the maturation of myeloid dendritic cells (mDCs)
4
. Additionally, maturation markers like CD80 are activated upon treatment of dendritic cells (DCs) with poly (I:C).

Applications

Poly (I:C) can be used for the activation of immune cells, such as
  1. Dendritic cells (DCs).
  2. Antiviral activity.
  • Selected references

    1. Ranjith-Kumar, C. T. et al. (2007) Biochemical and functional analysis of the human Toll-Like Receptor 3 ectodomain. J. Biol. Chem. 282(10): 7668-7678
    2. Alexopoulou, L. et al. (2001) Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3. Nature 413(6587): 732-738
    3. Kato, H. et al. (2008) Length-dependent recognition of double-stranded ribonucleic acids by retinoic acid inducible gene-I and melanoma differentiation associated gene 5. J. Exp. Med. 205(7): 1601-1610
    4. Rouas, R. et al. (2004) Poly (I:C) used for human dendritic cell maturation preserves their ability to secondarily secrete bioactive IL-12. Int. Immunol. 16(5): 767-773
Product options: 2
130-112-563

Poly (I:C)

50 mg
USD 230.00
130-112-562

Poly (I:C)

5×50 mg
USD 600.00