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Human purified T cells were isolated using the Pan T Cell Isolation Kit and activated for 48 hours using the T Cell TransAct™ (titer 1:100) in TexMACS™ Medium supplemented with Human IL-2 (20 IU/mL). The negative control experiment was performed without adding the T Cell TransAct. Cells were fluorescently stained using CD25-PE and CD69-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. CD4-VioBlue®
was used for selection of T helper cells and CD8-VioGreen™ was used for selection of cytotoxic T cells. Dead cells and debris were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
T cells after activation
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