PepTivator EBV Consensus, human

PepTivator EBV Consensus, human

PepTivator
®
EBV Consensus is a pool of 43 lyophilized MHC class I and class II restricted peptides, derived from 13 different proteins from Epstein-Barr virus (EBV).
PepTivator peptide pools have been developed for the efficient
in vitro
stimulation of antigen-specific T cells in various applications.

Background information

EBV is a human γ-herpesvirus with B cell growth–transforming ability and lymphomagenic potential. In healthy individuals, EBV typically establishes a persistent latent infection.
The PepTivator EBV Consensus pool contains 43 peptides of 8-20 aa in length. 32 of these peptides are MHC class I resticted, 11 peptides are MHC class II restricted.
The sequences of these peptides were derived from the following 13 different lytic and latent EBV proteins:
LMP2a; BRLF1; BMLF1 (EB2); BNLF1 (LMP1); BERF3 (EBNA6); BERF1 (EBNA3); BERF2 (EBNA4); BALF2 (DNBI); BMRF1; BZLF1; BNRF1 (MTP); EBNA1; BLLF1 (gp350).
The peptides are restricted for the following HLA-molecules:
HLA-A*02, -A*03, -A*11, -A*24, -A*26, -B*07, -B*08, -B*15, -B*18, -B*27, -B*35, -B*40, -B*4402, -DR

Applications

The
in vitro
stimulation of EBV-specific T cells with PepTivator EBV Consensus causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of EBV-specific T cells.
  • Detection and analysis of EBV-specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
  • Isolation of viable EBV-specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of EBV-specific CD4+ and CD8+ effector/memory T cells from naive T cell populations.
  • Pulsing of antigen-presenting cells for research on dendritic cell vaccination.
Product options: 3

Product information

Size
Order no.
Price

PepTivator EBV Consensus - premium grade, human

  • For research use only
for
stimulation of
10
8
cells
130-099-764
USD 215.00

PepTivator EBV Consensus - premium grade, human

  • For research use only
for
stimulation of
10
9
cells
130-103-462
USD 1,275.00

PepTivator EBV Consensus (HT) - premium grade, human

  • For research use only
for 96
tests
130-119-472
USD 385.00

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