Name: PepTivator BKV VP1, human
Availability: InStock

Product overview

PepTivator

^®

BKV VP1 is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the human BK virus (BKV) VP1 capsid protein (UniProt ID: P14996).

The

in vitro

stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Detailed product information

Background information

BKV is a ubiquitous polyomavirus. After primary infection BKV persists in a latent state. Virus reactivation might occur in immunodeficiency or during immunosuppression. VP1 is the major capsid protein of BKV, expressed late in the lytic cycle.

Downstream applications

PepTivator BKV VP1 – research grade has been specifically developed for efficient

in vitro

stimulation of BKV VP1–specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4

⁺

and CD8

⁺

T cells in various applications, including:

Detection and analysis of BKV VP1–specific effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
Isolation of viable BKV VP1–specific CD4⁺ T cells with the CD154 MicroBead Kit, or of CD4⁺ and CD8⁺ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
Generation of BKV VP1–specific effector/memory T cells from naive T cell populations.
Pulsing of antigen-presenting cells.

Product data and images

Figure 1

Detection and isolation of viable BKV–specific T cells by intracellular staining using MACS^® Rapid Cytokine Inspector (CD4/CD8 T Cell) Kit (# 130-097-343)

From a BKV

⁺

donor, 10

⁶

human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator

^®

BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). PBMCs stimulated with PepTivator CEF MHC Class I Plus (# 130-098-426) were used as positive control.

The cells were stained with the CD4/CD8 T Cell Detection Cocktail and Rapid Cytokine Inspector Anti-IFN-γ-PE as described and were analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris, monocytes, and B cells were excluded from the analysis. IFN-γ expression was analyzed for CD4

⁺

and CD8

⁺

T cells.