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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Detection and isolation of viable BKV–specific T cells by intracellular staining using MACS® Rapid Cytokine Inspector (CD4/CD8 T Cell) Kit (# 130-097-343) From a BKV + donor, 10 6 human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator ® BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). PBMCs stimulated with PepTivator CEF MHC Class I Plus (# 130-098-426) were used as positive control. The cells were stained with the CD4/CD8 T Cell Detection Cocktail and Rapid Cytokine Inspector Anti-IFN-γ-PE as described and were analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris, monocytes, and B cells were excluded from the analysis. IFN-γ expression was analyzed for CD4 + and CD8 + T cells. |
A: | ||
Unstimulated control | Stimulated sample | |
B: | ||
Unstimulated control | Stimulated sample | |
C: | ||
Unstimulated control | Stimulated sample | Positive control |
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